PRPF4 Antibody / U4/U6 small nuclear ribonucleoprotein Prp4

• Description:

  U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. he protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  O43172

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E. coli-derived recombinant human protein (amino acids R5-H461) was used as the immunogen for the PRPF4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS, IHC-P, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the PRPF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PRPF4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PRPF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PRPF4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of FFPE human U-2 OS cells with PRPF4 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.