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Prostasin Antibody / PRSS8

CAT: 0800-RQ8101Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8101Size:100 µg
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Description
Prostasin is a protein that in humans is encoded by the PRSS8 gene This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q16651
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids Q59-R322) was used as the immunogen for the Prostasin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Prostasin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Prostasin antibody is available for research use only.
Storage Conditions
After reconstitution, the Prostasin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Prostasin antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Prostasin antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Prostasin antibody.

UniProtKB · Q16651

Prostasin

PRSS8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16651
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRSS8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
B4DWP2, Q9UCA3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

SignalTransmembraneTransmembrane helix
Product Datasheet
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