PRPF8 Antibody / Pre-mRNA-processing-splicing factor 8

• Description:

  Pre-mRNA-processing-splicing factor 8 is a protein that in humans is encoded by the PRPF8 gene. Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

• Specifications:

  Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q6P2Q9

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  E. coli-derived recombinant human protein (amino acids E433-H2166) was used as the immunogen for the PRPF8 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the PRPF8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PRPF8 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PRPF8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PRPF8 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) human HeLa, 2) human SH-SY5Y and 3) mouse NIH 3T3 cell lysate with PRPF8 antibody. Predicted molecular weight ~274 kDa.