Bulk Human IgG4 (IB4) Isotype Control - Extremely Low Endotoxin

• Background:

  Recombinant IgG4 human antibody

• Specifications:

  This Human IgG4 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG4 antibody is suitable for in vivo applications. This Human IgG4 isotype control contains the S228P mutation

• Host:

  CHO

• Species Reactivity:

  Human

• Target:

  IgG4

• Clone:

  IB4

• Applications:

  Flow Cytometry, Functional Assays

• Purification Method:

  This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

• Concentration:

  5mg/mL

• Endotoxin:

  <1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.