Anti-Mouse CD317 (927) In Vivo Antibody - Low Endotoxin

• Specifications:

  Clone 927 activity is directed against murine CD317 (BST2; PDCA-1).

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD317

• Isotype:

  IgG2a kappa

• Clone:

  927

• Applications:

  Depletion, Functional Assays, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  <1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  Sterile, preservative-free, solution in PBS. BSA and Azide free.

• Antigen Distribution:

  Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4