Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin

• Specifications:

  Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v.

• Host:

  Mouse

• Species Reactivity:

  Mouse

• Target:

  MHC Class I

• Isotype:

  IgG2a κ

• Clone:

  AF6-88.5

• Applications:

  Functional Assays, Flow Cytometry, IHC Frozen, Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  <1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  Sterile, preservative-free, solution in PBS. BSA and Azide free.

• Antigen Distribution:

  The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

• CAS Number:

  9007-83-4