Anti-Mouse Ly49C (4LO3311) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.

• Specifications:

  4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1). 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.

• Product Name Alternative:

  Ly-49 inhibitory receptors C

• Host:

  Mouse

• Species Reactivity:

  Mouse

• Target:

  Ly49C

• Isotype:

  IgG3 Kappa

• Clone:

  4LO3311

• Applications:

  Depletion, Flow Cytometry, Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  Sterile, preservative-free, solution in PBS. BSA and Azide free.

• Antigen Distribution:

  Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG3-Isotype-Control-for-In-Vivo-Low-Endotoxin-MG3-35-ICH2249

• CAS Number:

  9007-83-4