Anti-Mouse CD49d (PS/2) In Vivo Antibody - Low Endotoxin

• Background:

  Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.

• Specifications:

  PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.

• Product Name Alternative:

  VLA-4α, ITGA4, Integrin α4

• Host:

  Rat

• Species Reactivity:

  Mouse, Human

• Target:

  CD49d

• Isotype:

  IgG2b Kappa

• Clone:

  PS/2

• Applications:

  Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  <1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  Sterile, preservative-free, solution in PBS. BSA and Azide free.

• Antigen Distribution:

  CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4