Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin

Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin

• Background: According to surface plasmon resonance, 9E9 has strong reactivity to FcγRIV as well as low level binding to FcγRII and FcγRIII. In vivo, 9E9 binds and blocks FcγRIII only when 9E9 first binds FcγRIV on the same effector cell, resulting in concurrent inhibition of FcγRIII and FcγRIV. Native 9E9 binds to FcγRII and FcγRIII via the Fc. Blocking studies with 9E9 show that FcγRIV is necessary for IgG2a and IgG2b mediated platelet clearance in vivo. Additionally, blocking FcγRIV with 9E9 reduces B-cell depletion. 9E9 also interferes with immune complex binding to FcγRIV and can block FcγRIII on macrophages and neutrophils.
• Specifications: 9E9 activity is primarily directed against mouse CD16.2 / FcγRIV but can also bind and block FcγRIII in vivo.
• Product Name Alternative: FcγRIV
• CAS Number: 9007-83-4
• Host: Armenian Hamster
• Species Reactivity: Mouse
• Target: CD16.2
• Isotype: IgG
• Clone: 9E9
• Applications: Blocking, Flow Cytometry
• Purification Method: This monoclonal antibody was purified using Protein A
• Concentration: ≥ 2.0 mg/mL
• Endotoxin: <1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
• Formulation: Sterile, preservative-free, solution in PBS. BSA and Azide free.
• Antigen Distribution: FcγRIV is expressed on the cell membrane of splenic and bone marrow dendritic cells, monocytes, and macrophages as well as peripheral blood monocytes, neutrophils, thioglycollate-elicited macrophages, and myeloid cells. FcγRIV is absent from lymphoid populations, T cells, B cells, NK cells, and other granulocytes.
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
• Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251