Anti-Mouse CD24 (M1/69) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways as well as a crucial role in cell selection and maturation during hematopoiesis.

• Specifications:

  Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52.

• Product Name Alternative:

  Heat Stable Antigen, Nectadrin, Ly-52

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD24

• Isotype:

  IgG2b Kappa

• Clone:

  M1/69

• Applications:

  Inhibitor, ELISA Indirect, Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, Immunoprecipitation, Western Blot

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  Sterile, preservative-free, solution in PBS. BSA and Azide free.

• Antigen Distribution:

  CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4