Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naïve T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies. RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo. RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(−) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity

• Specifications:

  RM27-3E5 activity is directed against mouse CD27

• Product Name Alternative:

  CD27L receptor, Tnfrsf7

• UniProt:

  P41272

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD27

• Isotype:

  IgG1 Kappa

• Clone:

  RM27-3E5

• Applications:

  Functional Assays, Flow Cytometry, ImmunoPrecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  ≥ 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

• Antigen Distribution:

  CD27 is expressed on T cells, B cells, and NK cells

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://ichor.bio/product/rat-igg1-isotype-control-for-in-vivo-low-endotoxin-gl113-ich2246/

• CAS Number:

  9007-83-4