Mouse Anti-Mouse PD-1 (RMP1-14) Recombinant Antibody D265A

Mouse Anti-Mouse PD-1 (RMP1-14) Recombinant Antibody D265A

• Background: CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
• Specifications: Mouse anti-PD-1 antibody (RMP1-14) is a recombinant antibody where the original Rat IgG2a backbone of clone RMP1-14 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse PD-1
• Product Name Alternative: Programmed cell death protein 1, Pdcd1, CD279
• CAS Number: 9000-83-3
• UniProt: Q02242
• Host: CHO
• Species Reactivity: Mouse
• Target: PD-1
• Isotype: IgG1
• Clone: RMP1-14
• Applications: Western Blot, Blocking, Flow Cytometry, Functional Assays
• Purification Method: This monoclonal antibody was purified using Protein A
• Concentration: 1.0 - 5.0 mg/mL
• Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE and HPLC
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: Mouse Anti-mouse PD-1 antibody (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.
• Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
• Antigen Distribution: Subset of double negative thymocytes, activated T and B cells
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
• Isotype Control: https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247