Anti-Mouse TCR Beta (H57-597) Antibody In Vivo Antibody - Low Endotoxin

Anti-Mouse TCR Beta (H57-597) Antibody In Vivo Antibody - Low Endotoxin

• Background: TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival.
• Specifications: Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
• Product Name Alternative: TCR-β chain, TCR-β, β-TCR
• CAS Number: 9007-83-4
• Host: Armenian Hamster
• Species Reactivity: Mouse
• Target: TCR Beta
• Isotype: IgG
• Clone: H57-597
• Applications: Flow Cytometry, Costimulatory, Depletion, Immunoprecipitation, IHC (Frozen), IHC (Paraffin), CODEX
• Purification Method: This monoclonal antibody was purified using Protein A
• Concentration: ≥ 5.0 mg/mL
• Endotoxin: <1.0 EU/mg as determined by the LAL method
• Purity: ≥95% monomer by analytical SEC, >95% by SDS Page
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
• Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
• Antigen Distribution: TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
• Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251