Anti-Mouse TNF Alpha (TN3-19.12) In Vivo Antibody - Low Endotoxin

• Background:

  The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha

• Specifications:

  Anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) recognizes Mouse TNFa

• Product Name Alternative:

  Cachectin, TNFa, Tumor necrosis factor ligand superfamily member 2, Tnfsf2

• UniProt:

  P06804

• Host:

  Armenian Hamster

• Species Reactivity:

  Mouse

• Target:

  TNF alpha

• Isotype:

  IgG

• Clone:

  TN3-19.12

• Applications:

  Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, Blocking

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

• CAS Number:

  9007-83-4