Anti-Mouse IFN Alpha (TIF-3C5) In Vivo Antibody - Ultra-Low Endotoxin

• Specifications:

  Anti-IFN alpha In Vivo Antibody - Ultra-Low Endotoxin (TIF-3C5) recognizes an epitope on Mouse IFNalpha and binds specifically to murine IFNa species (IFN-alpha-A, -1, -4, -5, -11. and -13) and does not bind murine murine IFNg or IFNb

• Product Name Alternative:

  IFNa, interferon alpha

• UniProt:

  P01572

• Host:

  Armenian Hamster

• Species Reactivity:

  Mouse

• Target:

  IFN alpha

• Isotype:

  IgG

• Clone:

  TIF-3C5

• Applications:

  Western Blot, Functional Assays

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) is stable when stored at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Functional Assay: In vitro activity of TIF-3C5 - inhibits IFNalpha -induced (IFNalpha-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNA (IFNA-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNA-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNalpha-A, -1, -4, -5, -11 and 13) and natural IFNalpha can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

• CAS Number:

  9007-83-4