Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Ultra-Low Endotoxin

• Specifications:

  Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

• Product Name Alternative:

  IFN-b, IFN-beta, Type-1 interferon, Interferon beta

• UniProt:

  P01575

• Host:

  Mouse

• Species Reactivity:

  Mouse

• Target:

  IFN beta

• Isotype:

  IgG2a

• Clone:

  HDB-4A7

• Applications:

  Western Blot, Blocking, Functional Assays

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Functional Assay: In Vitro Activity of HDB-4A7 - inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). - blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. - neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV In Vivo Activity of HDB-4A7 - circulates with a half-life of ~ 14 days. - blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

• CAS Number:

  9007-83-4