Anti-Mouse IL-1b (B122) In Vivo Antibody - Low Endotoxin

• Background:

  Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes. IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease. The B122 antibody has been reported to neutralize the bioactivity of natural or recombinant IL-1β.

• Specifications:

  Anti-IL-1b In Vivo Antibody - Low Endotoxin (B122) recognizes Mouse IL-1B

• Product Name Alternative:

  Interleukin-1 beta, IL-1B

• UniProt:

  P10749

• Host:

  Armenian Hamster

• Species Reactivity:

  Mouse

• Target:

  IL-1b

• Isotype:

  IgG

• Clone:

  B122

• Applications:

  Neutralization, Immunoprecipitation, Western Blot, ELISA

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-IL-1b In Vivo Antibody - Low Endotoxin (B122) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells and many other B cells, T cells, related monocytes

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

• CAS Number:

  9007-83-4