Anti-Mouse Dendritic Cells (33D1) In Vivo Antibody - Low Endotoxin

• Background:

  Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions.

• Specifications:

  Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells

• Product Name Alternative:

  DCIR2

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  Dendritic Cells

• Isotype:

  IgG2b

• Clone:

  33D1

• Applications:

  Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  0.5 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles

• Formulation:

  No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

• Antigen Distribution:

  Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4