Anti-Mouse CTLA-4 (UC10-4F10-11) In Vivo Antibody - Low Endotoxin

• Background:

  CD152, also known as CTLA-4 or Ly-56 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

• Specifications:

  Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) recognizes an epitope on Mouse CD152

• Product Name Alternative:

  Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

• UniProt:

  P09793

• Host:

  Armenian Hamster

• Species Reactivity:

  Mouse

• Target:

  CTLA-4

• Isotype:

  IgG1 Kappa

• Clone:

  UC10-4F10-11

• Applications:

  Flow Cytometry, Western Blot, Functional Assays, Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) is stable when stored at 2-8°C. Do not freeze.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.4, without BSA or sodium azide added.

• Antigen Distribution:

  The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Functional Assay: Clone UC10-4F10-11 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

• CAS Number:

  9007-83-4