Anti-CTLA-4 (9H10) In Vivo Antibody - Low Endotoxin

• Background:

  CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. Clone 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA4 binding, allowing for CD28 binding.

• Specifications:

  Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9H10) recognizes an epitope on Mouse CTLA-4

• Product Name Alternative:

  Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

• UniProt:

  P09793

• Host:

  Syrian Hamster

• Species Reactivity:

  Mouse

• Target:

  CTLA-4

• Isotype:

  IgG

• Clone:

  9H10

• Applications:

  Western Blot, Blocking, ELISA, Flow Cytometry

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9H10) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  Activated T cells

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• CAS Number:

  9007-83-4