Anti-Mouse CD172a (P84) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  Signal regulatory protein alpha (SIRPalpha), also known as CD172a, is a regulatory membrane type I glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.

• Specifications:

  Anti-CD172a In Vivo Antibody - Ultra-Low Endotoxin (P84) recognizes an epitope on Mouse CD172a

• Product Name Alternative:

  Sirpa, Sirp-alpha-1, SHP substrate 1, SHPS-1, Shps1, Bit, p84, MyD-1 antigen, Myd1, Ptpns1

• UniProt:

  P97797

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD172a

• Isotype:

  IgG1 Kappa

• Clone:

  P84

• Applications:

  Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD172a In Vivo Antibody - Low Endotoxin (P84) is stable when stored at 2-8°C. Do not freeze.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  Monocytes, macrophages, myeloid cells, neuronal tissue

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Blocking: Reported applications in literature include: blocking SIRPalpha interaction with CD47 & in vivo blocking of dendritic cell migration. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246

• CAS Number:

  9007-83-4