Anti-Mouse CD40 (FGK4.5) In Vivo Antibody - Low Endotoxin

• Background:

  CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD40) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

• Specifications:

  Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) recognizes an epitope on Mouse CD40

• UniProt:

  P27512

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD40

• Isotype:

  IgG2a

• Clone:

  FGK4.5

• Applications:

  Western Blot, Functional Assays, Flow Cytometry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  <0.5 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added

• Antigen Distribution:

  B cells, basal epithelial cells, macrophages, follicular dendritic cells, and endothelial cells

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4