Anti-Mouse CD11a (FD441.8) In Vivo Antibody - Low Endotoxin

• Specifications:

  Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) recognizes an epitope on Mouse CD11a

• Product Name Alternative:

  LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL, Ly-15

• UniProt:

  P24063

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD11a

• Isotype:

  IgG2b

• Clone:

  FD441.8

• Applications:

  Western Blot, Functional Assays, Flow Cytometry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Functional Assay: The antibody inhibits ConA-facilitated lysis, but does not affect PHA-facilitated lysis. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4