Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

• Background:

  CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.

• Specifications:

  Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4

• Product Name Alternative:

  T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

• UniProt:

  P06332

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD4

• Isotype:

  IgG2b Kappa

• Clone:

  GK1.5

• Applications:

  Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  ≥ 2.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4