Bulk Anti-Human MHC Class I (W6/32) Antibody

• Background:

  MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surveillance.

• Specifications:

  Anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C

• Product Name Alternative:

  Major Histocompatibility Class I, MHC class I

• Host:

  Mouse

• Species Reactivity:

  Human

• Target:

  MHC Class I

• Isotype:

  IgG2a

• Clone:

  W6/32

• Applications:

  Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  No carrier proteins or preservatives in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl.

• Antigen Distribution:

  This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

• CAS Number:

  9007-83-4