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4E-T Antibody / EIF4ENIF1

CAT: 0800-RQ7948Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7948Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
Q9NRA8
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E. coli-derived recombinant human protein (amino acids D13-Q985) was used as the immunogen for the 4E-T antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the 4E-T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This 4E-T antibody is available for research use only.
Storage Conditions
After reconstitution, the 4E-T antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the 4E-T antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= 4E-T antibody.

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