OGDH Antibody / 2-oxoglutarate dehydrogenase

• Description:

  Alpha-ketoglutarate dehydrogenase also known as 2-oxoglutarate dehydrogenase E1 component, mitochondrial is an enzyme that in humans is encoded by the OGDH gene. This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO (2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q02218

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E. coli-derived recombinant human protein (amino acids M1-S1023) was used as the immunogen for the OGDH antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the OGDH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This OGDH antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the OGDH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the OGDH antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human Daudi cells with OGDH antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= OGDH antibody.