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RANBP2 Antibody

CAT: 0800-RQ7790Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7790Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RAN binding protein 2 (RANBP2) is protein which in humans is encoded by the RANBP2 gene. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. And the encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P49792
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids N906-D2744) was used as the immunogen for the RANBP2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RANBP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RANBP2 antibody is available for research use only.
Storage Conditions
After reconstitution, the RANBP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RANBP2 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic (vesicles)
Image Legend
IHC staining of FFPE human breast cancer tissue with RANBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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