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FLAG-tag Polyclonal Antibody

CAT: 0965-JOT-AP15612-01Size: 20 µLDry Ice: NoHazardous: No
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CAT#:0965-JOT-AP15612-01Size:20 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
CAS Number
9007-83-4
Synonyms
Flag-Tag DDDDK TAG, DDDDK-TAG; DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Host
Rabbit
Reactivity
Species independent
Immunogen
DDDDK synthetic peptide conjugated to KLH.
Clonality
Polyclonal
Applications
WB, ELISA
Stability
-20°C for 1 year
Concentration
1 mg/ml
Antibody Type
Primary antibody
Isotype
IgG

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