Products for Research Use Only

C4, Mouse, mAb 16D2 Antibody

CAT: 0013-GTR18086798Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18086798Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse monoclonal antibody to C4
Product Name Alternative
Complement component 4
Reactivity
Mouse
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components
Clonality
Monoclonal
Conjugation
Unconjugated
Dilution
For Immuno Assays, Immuno Fluorescence, Immuno Precipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: A non-reduced sample treatment and SDS-Page was used. The band size is 200 kDa (Ref.4) . IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20 (Ref.2) . IF: Spleens were snap frozen and stored at -80°C until cryosections were cut. Sections (5 μm) were fixed for 4 min in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS, and PBS. Biotinylated 16D2 was visualized using avidin-PE (BD PharMingen, San Diego, CA) (Ref.2) .
Tested Applications
IA, IF, IP, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a