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Anti-Human IL-6

CAT: 0013-GTR18085607Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18085607Size:100 µL
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Description
Rabbit polyclonal antibody to IL6
Reactivity
Human
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified
Concentration
1ug/ul
Dilution
This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC) . PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.
Purity
Purified
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC) . PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
Isotype
IgG

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