Recombinant Rat Granulocyte- Macrophage Colony Stimulating Factor (rrGM-CSF)

• Description:

  GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

• Endotoxin:

  Less than 1EU/mg of rr GM-CSF as determined by LAL method.

• Purity:

  >98% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  The ED50 was determined by the dose-dependent stimulation of the proliferation of Murine FDC-P1 cells is < 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 108 units/mg.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.

• Molecular Weight:

  Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.

• Storage Conditions:

  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China

• CAS Number:

  9000-83-3

• AA Sequence:

  MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK