Recombinant Human BAFF Receptor (rHuBAFF-R)

• Description:

  BAFF Receptor (BAFF-R), a member of the TNFR superfamily, is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF-R is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B) . BAFF-R/BAFF signaling plays a critical role in B cell survival and maturation.

• Endotoxin:

  Less than 1EU/mg of rHuBAFF-R as determined by LAL method.

• Purity:

  >95% by reduced SDS-PAGE analyses.

• Bioactivity:

  Fully biologically active when compared to standard. Determined by its ability to block BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 1.0-5.0 μg/ml in the presence of 1.0μg/ml of human soluble BAFF, corresponding to a Specific Activity of > 200 IU/mg.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

• Molecular Weight:

  Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

• Storage Conditions:

  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 8.0, 500mM NaCl.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China

• CAS Number:

  9000-83-3

• AA Sequence:

  MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG