CTCF Recombinant Rabbit mAb

• Background:

  This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

• Structure Composition:

  Store at -20°C. Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

• Product Name Alternative:

  MRD21

• Swiss Prot:

  P49711

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide of human CTCF

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF, FC

• Purity:

  Affinity Purification

• Modification:

  Unmodification

• Molecular Weight:

  140 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of extracts from HeLa, HCT116 cells using BS49493 at 1: 3000.

• Host or Source:

  Rabbit

• CAS Number:

  9000-83-3