Phospho-Topoisomerase II alpha (Thr1343) Recombinant Rabbit mAb

• Background:

  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

• Structure Composition:

  Store at -20°C. Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

• Product Name Alternative:

  TOP2; TP2A

• Swiss Prot:

  P11388

• Reactivity:

  Human

• Immunogen:

  A synthetic phosphopeptide corresponding to residues surrounding Thr1343 of human Topoisomerase II alpha

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF

• Purity:

  Affinity Purification

• Modification:

  Phosphorylated

• Molecular Weight:

  174 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of extracts from HeLa cells at 1:1000.

• Host or Source:

  Rabbit

• CAS Number:

  9000-83-3