RNF7 polyclonal antibody

• Background:

  The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII) . The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B) . Alternatively spliced transcript variants encoding distinct isoforms have been reported.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  RNF7; CKBBP1; ROC2; SAG

• Swiss Prot:

  Q9UBF6

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein of human RNF7 (NP_055060.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC

• Dilution:

  WB,1:500 - 1:2000|IF/ICC,1:20 - 1:50

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  Refer to figures

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Polyclonal Antibodies

• Applications Notes:

  Immunofluorescence analysis of MCF-7 cells using RNF7 antibody . Blue: DAPI for nuclear staining.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4