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DDIT3/CHOP polyclonal antibody

CAT: 0384-BS6814-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS6814-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DDIT3 / CHOP
Swiss Prot
P35638
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human DDIT3/CHOP (NP_004074.2) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IHC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
27KDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Polyclonal Antibodies
Applications Notes
Immunohistochemistry of paraffin-embedded human prostate cancer using DDIT3/CHOP Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Host or Source
Rabbit

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