COMT polyclonal antibody

• Background:

  Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT) . The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  COMT; HEL-S-98n

• Swiss Prot:

  P21964

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  RRecombinant fusion protein containing a sequence corresponding to amino acids 51-271 of human COMT. (NP_000745.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB IF/ICC

• Dilution:

  WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 28, 30 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  COMT polyclonal antibody detects endogenous levels of COMT protein.

• Applications Notes:

  Western blot analysis of lysates from THP-1 cells using COMT pAb at 1:700 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4