GLUL polyclonal antibody

• Background:

  Glutamine synthetase (GI Syn) forms a homooctamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Glutamine synthetase; GS; Glutamate decarboxylase; Glutamate--ammonia ligase; GLNS; Gl Syn

• Swiss Prot:

  P15104

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Glutamine Synthetase.

• Conjugation:

  Unconjugated

• Related Pathways:

  Glutamine Metabolism

• Applications:

  WB IHC IP FC

• Dilution:

  WB: 1:500-1:2000 IHC: 1:50-1:200 IP/FC: 1:50

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 49 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Glutamine Synthetase Antibody detects endogenous levels of total Glutamine Synthetase.

• Applications Notes:

  Western blot analysis of Glutamine Synthetase expression in (1) Jurkat cell lysate; (2) HeLa cell lysate.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4