CBFA2T2 polyclonal antibody

• Background:

  In acute myeloid leukemia, especially in the M2 subtype, the t (8;21) (q22; q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein CBFA2T2; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85; CBFA2T2; EHT, MTGR1

• Swiss Prot:

  O43439

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human CBFA2T2.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB 1:500 - 1:2000 IF 1:50 - 1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 67kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CBFA2T2 polyclonal antibody detects endogenous levels of CBFA2T2 protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using CBFA2T2 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4