NACA polyclonal antibody

• Background:

  This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC) . This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  HSD48; NAC-alpha; NACA1; skNAC

• Swiss Prot:

  Q13765

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein of human NACA

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB 1:2000 - 1:5000 IHC 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  37kDa, 39kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  NACA polyclonal antibody detects endogenous levels of NACA protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using NACA antibody at 1:1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4