CDC14A polyclonal antibody

• Background :

  In budding yeast, the Cdc14a phosphatase activates mitotic exit by dephosphorylation of specific cyclin-dependent kinase (Cdk) substrates and seems to be regulated by sequestration in the nucleolus until its release during mitosis. Human Cdc14a phosphatase is highly similar to Saccharomyces cerevisiae Cdc14 and is a member of the dual specificity protein Tyrosine phosphatase family. It interacts with and dephosphorylates tumor suppressor protein p53 and may regulate the function of p53. In addition, Cdc14a dephosphorylates hCdh1 and activates APCCdh1. Cdc14a phosphatase plays a role in the regulation of the centrosome cycle, mitosis and cytokinesis, thereby influencing chromosome partitioning and genomic stability in human cells. Deregulated human Cdc14a phosphatase disrupts centrosome separation and chromosome segregation.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Dual specificity protein phosphatase CDC14A; CDC14 cell division cycle 14 homolog A; CDC14A

• Swiss Prot :

  Q9UNH5

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 150-200 of Human CDC14A.

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 66 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CDC14A polyclonal antibody detects endogenous levels of CDC14A protein.

• Applications Notes :

  Western blot (WB) analysis of CDC14A polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4