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USP15 polyclonal antibody

CAT: 0384-BS61734-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS61734-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP15 (ubiquitin specific peptidase 15), also known as UNPH4, is a member of the peptidase C19 family of proteins. Expressed in kidney, liver, placenta, ovary, lung, thymus, heart and skeletal muscle, USP15 localizes to the cytoplasm and the nucleus, contains one DUSP domain and functions as a deubiquitinating enzyme that cleaves ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. Via its DUSP domain, USP15 plays a role in the regulation of the COP9 signalosome (CSN) complex. Three isoforms exist for USP15 due to alternative splicing events.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ubiquitin carboxyl-terminal hydrolase 15; Deubiquitinating enzyme 15; Ubiquitin thioesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4; USP15; KIAA0529
Swiss Prot
Q9Y4E8
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 80-130 of Human USP15.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
USP15 polyclonal antibody detects endogenous levels of USP15 protein.
Applications Notes
Western blot (WB) analysis of USP15 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit

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