SSH3 polyclonal antibody

• Background:

  Slingshot homolog 3 (SSH3) is a protein phosphatase that modifies actin cytoskeleton dynamics via cofilin dephosphorylation. Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms. The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3. The slingshot homologs (SSH1, SSH2, and, to a lesser extent, SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization. SSH3 is widely expressed in epithelial tissues, and has been found to be non-essential for viability and fertility in knockout mice. While its biological function remains elusive, phosphorylation at Ser37 of SSH3 has been identified in several phosphoproteomic studies.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein phosphatase Slingshot homolog 3; SSH-like protein 3; SSH-3L; hSSH-3L; SSH3; SSH3L

• Swiss Prot:

  Q8TE77

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding Human SSH3.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 90 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  SSH3 polyclonal antibody detects endogenous levels of SSH3 protein.

• Applications Notes:

  Western blot (WB) analysis of SSH3 pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4