RAB18 polyclonal antibody

Ras-related protein Rab-18, plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration. The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Ras-related protein Rab-18; RAB18

Q9NP72

Human, Mouse, Rat

Synthetic peptide, corresponding Human RAB18.

Unconjugated

WB

WB: 1:500~1:1000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 23 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

RAB18 polyclonal antibody detects endogenous levels of RAB18 protein.

Western blot (WB) analysis of RAB18 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2: Hela whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)