EID1 polyclonal antibody

CAT: 0384-BS61563-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS61563-01Size:50 µL
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
E1A-like inhibitor of differentiation-1 (EID-1), an acetyltransferase enzyme, binds both the retinoblastoma protein (Rb), a regulator of cell cycle and tissue specific transcription, and the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EID-1 inhibits cellular differentiation by blocking the histone acetyltransferase activity of p300. EID-1 also acetylates both histones and non-histone proteins such as NCOA3 co-activator. By acetylating histones, EID-1 gives a specific tag for transcriptional activation. In addition to binding Rb and p300, EID-1 also binds to phosphorylated CREB protein, mediating cAMP gene regulation. EID-1 augments the activity of phosphorylated CREB and activates transcription of cAMP responsive genes as a co-activator.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
EP300-interacting inhibitor of differentiation 1; 21 kDa pRb-associated protein; CREBBP/EP300 inhibitory protein 1; E1A-like inhibitor of differentiation 1; EID-1
Swiss Prot
Q9Y6B2
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 70-114 of Human EID1
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Modification
Unmodification
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EID1 polyclonal antibody detects endogenous levels of EID1 protein.
Applications Notes
Western blot (WB) analysis of EID1 polyclonal antibody at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:HCC827 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug)
Host or Source
Rabbit

Popular Products