EID1 polyclonal antibody

• Background :

  E1A-like inhibitor of differentiation-1 (EID-1), an acetyltransferase enzyme, binds both the retinoblastoma protein (Rb), a regulator of cell cycle and tissue specific transcription, and the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EID-1 inhibits cellular differentiation by blocking the histone acetyltransferase activity of p300. EID-1 also acetylates both histones and non-histone proteins such as NCOA3 co-activator. By acetylating histones, EID-1 gives a specific tag for transcriptional activation. In addition to binding Rb and p300, EID-1 also binds to phosphorylated CREB protein, mediating cAMP gene regulation. EID-1 augments the activity of phosphorylated CREB and activates transcription of cAMP responsive genes as a co-activator.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  EP300-interacting inhibitor of differentiation 1; 21 kDa pRb-associated protein; CREBBP/EP300 inhibitory protein 1; E1A-like inhibitor of differentiation 1; EID-1

• Swiss Prot :

  Q9Y6B2

• Reactivity :

  Human

• Immunogen :

  Synthetic peptide, corresponding to amino acids 70-114 of Human EID1

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

• Modification :

  Unmodification

• Molecular Weight :

  ~ 28 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  EID1 polyclonal antibody detects endogenous levels of EID1 protein.

• Applications Notes :

  Western blot (WB) analysis of EID1 polyclonal antibody at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:HCC827 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4