DUSP16 polyclonal antibody

• Background :

  DUSP16/MKP7 is a negative regulator of the JNK/SAPK family of stress-activated MAP kinases. It inhibits JNK-mediated signaling events by dephosphorylating threonine and tyrosine residues within the activation loop of JNK proteins, effectively preventing further activation of downstream effectors. DUSP16/MKP7 expression has been shown to be upregulated after oxidative stress, presumably as a means of supressing JNK activity in order to return the cells to a homeostatic state. DUSP16 is normally turned over at a high-rate in most cells, but the stability of the protein can be enhanced by Erk1/2-mediated phosphorylation on Ser446, indicating that activation of mitogenic signaling pathways can supress stress-response pathways via stabilization of a JNK phosphatase . Despite demonstrating a substrate preference towards JNK proteins, DUSP16/MKP7 has been shown to interact with other MAPK family members (Erk1/2, p38 MAPKs) as well as scaffolding proteins that may coordinate its activity and specificity.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Dual specificity protein phosphatase 16; Mitogen-activated protein kinase phosphatase 7; MAP kinase phosphatase 7; MKP-7

• Swiss Prot :

  Q9BY84

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 577-621 of Human DUSP16

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

• Modification :

  Unmodification

• Molecular Weight :

  ~ 73 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  DUSP16 polyclonal antibody detects endogenous levels of DUSP16 protein.

• Applications Notes :

  Western blot (WB) analysis of DUSP16 polyclonal antibody at 1:500 dilution Lane1:The Kidney tissue lysate of Rat (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4