RIPK3 polyclonal antibody

• Background:

  The death domain is a cytoplasmic domain of approximately 80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS. Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2. However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis. RIP3 can be expressed as two splice variants, RIP3∫ and RIP3©, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus. Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; RIPK3; RIP3

• Swiss Prot:

  Q9Y572

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human RIPK3.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Apoptosis, Necrotic Cell Death

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 57 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  RIPK3 polyclonal antibody detects endogenous levels of RIPK3 protein.

• Applications Notes:

  Western blot (WB) analysis of RIPK3 polyclonal antibody at 1:500 dilution Lane1:Panc1 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4