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UBE2U polyclonal antibody

CAT: 0384-BS60597-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS60597-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ubiquitin-conjugating enzyme E2 U; Ubiquitin carrier protein U; Ubiquitin-protein ligase U; UBE2U
Swiss Prot
Q5VVX9
Reactivity
Human
Immunogen
A synthetic peptide corresponding to residues in Human UBE2U
Conjugation
Unconjugated
Related Pathways
Ubiquitin Proteasome
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
UBE2U polyclonal antibody detects endogenous levels of UBE2U protein.
Applications Notes
Western blot (WB) analysis of UBE2U polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate
Host or Source
Rabbit

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