DNAJB2 polyclonal antibody

• Background:

  The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of the J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. Two isoforms are produced due to alternative splicing.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  DnaJ homolog subfamily B member 2; DnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; HSJ-1; DNAJB2; HSJ1; HSPF3

• Swiss Prot:

  P25686

• Reactivity:

  Human, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human DNAJB2

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 36 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  DNAJB2 polyclonal antibody detects endogenous levels of DNAJB2 protein.

• Applications Notes:

  Western blot (WB) analysis of DNAJB2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:PC12 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4